The principle of flow cytometer and its clinical application 流式細(xì)胞儀工作原理及臨床應(yīng)用
The cell proliferation was observed with flow cytometer and mtt colorimetric test Atra明顯拮抗了雌激素對細(xì)胞增殖的正性調(diào)節(jié)作用。
3 . observe the binding of oligochitosan labeled with 2 - amac with macrophages under confocal laser microscope and analysis the fluorescence intensity by flow cytometer using the cell quest software 在激光掃描共聚焦顯微鏡下觀察2 -氨基吖啶酮標(biāo)記殼寡糖與巨噬細(xì)胞的結(jié)合,用流式細(xì)胞儀分析熒光強(qiáng)度。
After adding culture mediem of stably transfected jurkat cells to hepatocarcinoma cells , the binding specificity of the scfvs with hbsag was further confirmed by observation by fluorescence microscope , indirect immunofluorescence and flow cytometer analysis . prokaryotic expression plasmids ptat - ha - scfvs were successfully constructed 建系細(xì)胞培養(yǎng)上清與肝癌細(xì)胞作用后,經(jīng)熒光顯微鏡觀察、間接免疫熒光及流式細(xì)胞儀檢測進(jìn)一步確定表達(dá)的scfv融合蛋白具有與hbsag特異性結(jié)合的活性。
Cell compatibility of films is researched firstly , which will make a significant contribution to the using fha films in practice from development . cell cycle , measured by flow cytometer and mtt method , and cell growth curve are used to analyze the impact of material and the immersed medium to the multiplication of osteoblast - cell 通過mtt法,流式細(xì)胞儀測定細(xì)胞周期,以及細(xì)胞生長曲線的測定,分析研究了fha薄膜材料對成骨細(xì)胞增殖生長的影響以及材料的浸提液對細(xì)胞增殖的影響,通過細(xì)胞相對增殖活性的測定對fha薄膜進(jìn)行毒性評級。